Summary: This protocol details the isolation and in vitro maintenance of single hematopoietic stem cells (HSCs) in the absence of the bone marrow niche. The HSCs do not divide over 7 days and fully retain their long-term functional capacity in transplantation assays. Following hibernation culture. HSCs can be used to study quiescence exit and can be genetically manipulated as single c... https://www.spidertattooz.com/Detroit-Tigers-Throwback-Brush-Golfer-Hat/